Document 2105
 DOCN  M94A2105
 TI    The host cell antigenicity determines the cell membrane proteins on
       HIV-1 progeny.
 DT    9412
 AU    Capobianchi MR; Fais S; Abbate I; Castilletti C; Mercuri F; Cordiali Fei
       P; Ameglio F; Ficociello B; Dianzani F; Institute of Virology,
       University La Sapienza, Rome, Italy.
 SO    Int Conf AIDS. 1994 Aug 7-12;10(1):38 (abstract no. 127A). Unique
       Identifier : AIDSLINE ICA10/94370470
 AB    OBJECTIVE: Cell-derived membrane molecules, including HLA Class I and
       II, adhesion molecules such as ICAM-1, -2, -3 and LFA-1, as well as
       receptors for growth factors, are associated with the external surface
       of HIV-1. To test whether the expression of surface antigens by the host
       cells could influence the antigenic profile of the virus progeny, we
       compared the pattern of host cell derived proteins on HIV-1 progeny from
       cells with different surface markers. METHODS: Cells from T, B and
       monocytoid origin were used to replicate the HIV-1 and immobilized
       antibody capture (IAC) was used to identify the cell surface molecules
       incorporated in virions. Susceptible target cells were added to the IAC
       plates to verify the infectivity of the virus captured by MAbs. HIV-1
       laboratory strains with established cellular tropism, as well as primary
       isolates, were compared to verify the predictive value of such analysis
       on the cellular source of a given virus population. RESULTS: For the
       lymphotropic strain IIIB the cell adhesion receptors, as well as HLA
       antigens, which are expressed by all the cell lines used to propagate
       HIV-1, are constantly included in the virus progeny. On the contrary,
       the specific T, B and monocyte markers are incorporated in HIV-1 progeny
       according to the host cell origin. In keeping with these observations,
       the monocytic markers CD14 and CD68 are included in the monocytotropic
       HIV-1 Bal strain grown on macrophages, whereas these antigens are not
       present on this virus strain when grown on T blasts. The presence of
       cell specific markers is observed also in HIV-1 primary isolates grown
       in activated PBMC. DISCUSSION AND CONCLUSIONS: The host cell antigenic
       footprint on infectious HIV-1 could be used as marker of viral tropism.
       Preliminary data suggest that this approach can be applied to plasma
       samples for phenotyping circulating HIV-1 from viremic individuals,
       avoiding strain selection due to culture conditions. Host factors (i.e.
       cytokines), altering the cellular expression of membrane antigens (i.e.
       adhesion receptors), involved in the attachment of viral particles,
       could influence the ability of HIV-1 to infect both CD4 positive and
       negative cells.
 DE    Antigens, Surface/*ISOLATION & PURIF  B-Lymphocytes/MICROBIOLOGY  Cell
       Adhesion Molecules/ISOLATION & PURIF  Human  HIV Antigens/*ISOLATION &
       PURIF  HIV-1/*GENETICS  HLA Antigens/ISOLATION & PURIF
       Monocytes/MICROBIOLOGY  Phenotype  Predictive Value of Tests
       T-Lymphocytes/MICROBIOLOGY  Viral Envelope Proteins/ISOLATION & PURIF
       Virion/IMMUNOLOGY  Virus Replication  MEETING ABSTRACT

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).